藏鸡FTO基因表达的时空特性及与肌内脂肪含量的相关分析

doi:10.11843/j.issn.0366-6964.2017.07.003

Abstract

Abstract:

The aim of this study was to obtain the sequence of FTO gene of Tibetan chicken, analyze its sequence by bioinformatics, clarify its temporal-spatial expression characteristics, and reveal the relationship between the expression level of FTO gene and intramuscular fat content(IMF) at different developmental stages. Healthy Tibetan chickens in various ages(1-210 days of age) were selected, the tissue samples of heart, liver, spleen, lung, kidney, breast muscle, leg muscle and subcutaneous fat were collected for the total RNA extraction. The Reverse Transcription PCR(RT-PCR) was used to clone Tibetan chicken FTO gene sequence, the real-time quantitative PCR(qPCR) was used to detect the expression level of FTO gene in different tissues and developmental stages, and the correlation between the FTO mRNA expression and intramuscular fat content was analyzed. The results showed that the sequence of Tibetan chicken FTO gene was 1 585 bp (GenBank accession number:KY366175), containing 1 524 bp CDS, 35 bp 5' UTR and 26 bp 3' UTR, encoding 507 amino acids with a N-terminal domain and a C-terminal domain. There were 6 amino acid mutations between Tibetan chicken and Gallus gallus FTO amino acid sequences. FTO gene of Tibetan chicken was widely expressed in different tissues. The expression level of FTO gene was the highest in spleen, which was significantly higher than other tissues (P <0.01). FTO gene also kept a high expression level in adipose and lung tissues. The expression pattern of Tibetan chicken FTO gene in breast muscle and leg muscle were different, the expression level in breast muscle decreased gradually with the growth of Tibetan chicken, while in the leg muscle, the expression level increased first and then descended with the increasing age. The expression levels of Tibetan chickenFTO gene in different muscle tissues and different developmental stages presented diverse relationships with IMF content. The expression level of FTO in breast muscle of Tibetan chicken was negatively correlated with IMF content, and this correlation in hens was significantly stronger (P <0.05). In leg muscle, the expression level of FTO gene in cocks was positively correlated with IMF content(in period of 119-210 days, r=0.601, P <0.05), while in hens this relationship was opposite. The results suggest that FTO gene may play an important role in intramuscular fat deposition during the growth and development stage of Tibetan chicken. These results will facilitate the research on the structure and function of FTO gene in Tibetan chickens.

CLC Number:

S831.2

LIN Sen, LIN Ya-qiu, ZHU Jiang-jiang, XU Ya-ou, WANG Yong. Temporal-Spatial Characteristics of FTO Gene Expression in Tibetan Chicken and Its Correlation with Intramuscular Fat Content[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1191-1201.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2017.07.003

https://www.xmsyxb.com/EN/Y2017/V48/I7/1191

References

[1] WANG Y H, BYRNE K A, REVERTER A, et al. Transcriptional profiling of skeletal muscle tissue from two breeds of cattle[J]. Mamm Genome, 2005, 16(3):201-210.
[2] FRAYLING T M, TIMPSON N J, WEEDON M N, et al. A common variant in the FTO gene is associated with body mass index and predisposes to childhood and adult obesity[J]. Science, 2007, 316(5826):889-894.
[3] GERKEN T, GIRARD C A, TUNG Y C L, et al. The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase[J]. Science, 2007, 318(5855):1469-1472.
[4] HAUPT A, THAMER C, STAIGER H, et al. Variation in the FTO gene influences food intake but not energy expenditure[J]. Exp Clin Endocrinol Diabetes, 2009, 117(4):194-197.
[5] STRATIGOPOULOS G, PADILLA S L, LEDUC C A, et al. Regulation of Fto/Ftm gene expression in mice and humans[J]. Am J Physiol Regul Integr Comp Physiol, 2008, 294(4):R1185-R1196.
[6] FREDRIKSSON R, HÄGGLUND M, OLSZEWSKI P K, et al. The obesity gene, FTO, is of ancient origin, up-regulated during food deprivation and expressed in neurons of feeding-related nuclei of the brain[J]. Endocrinology, 2008, 149(5):2062-2071.
[7] FAWCETT K A, BARROSO I. The genetics of obesity:FTO leads the way[J]. Tends Genet, 2010, 26(6):266-274.
[8] FISCHER J, KOCH L, EMMERLING C, et al. Inactivation of the Fto gene protects from obesity[J]. Nature, 2009, 458(7240):894-898.
[9] CHURCH C, LEE S, BAGG E A L, et al. A mouse model for the metabolic effects of the human fat mass and obesity associated FTO gene[J]. PLoS Genet, 2009, 5(8):e1000599.
[10] CHURCH C, MOIR L, MEMURRAY F, et al. Overexpression of Fto leads to increased food intake and results in obesity[J]. Nat Genet, 2010, 42(12):1086-1092.
[11] 王玉峰. 鸡FTO基因:组织特异性表达、中枢核团定位、品种差异以及禁食的影响[D]. 南京:南京农业大学, 2009.
WANG Y F. Chicken FTO gene:tissue-specific expression, brain distribution, breed difference and effect of fasting[D]. Nanjing:Nanjing Agricultural University, 2009. (in Chinese)
[12] 林亚秋, 徐亚欧, 张润锋, 等. 藏鸡Chemerin和ChemR23基因时序表达及其与肌内脂肪含量的相关性研究[J]. 畜牧兽医学报, 2015, 46(8):1290-1299.
LIN Y Q, XU Y O, ZHANG R F, et al. Association analysis of sequential expression of Chemerin and ChemR23 with intramuscular fat contents in Tibetan chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2015, 46(8):1290-1299. (in Chinese)
[13] DE LUIS D A, ALLER R, CONDE R, et al. Relation of the rs9939609 gene variant in FTO with metabolic syndrome in obese female patients[J]. J Diabetes Complications, 2013, 27(4):346-350.
[14] WEN W Q, CHO Y S, ZHENG W, et al. Meta-analysis identifies common variants associated with body mass index in east Asians[J]. Nat Genet, 2012, 44(3):307-311.
[15] WARDLE J, CARNELL S, HAWORTH C M A, et al. Obesity associated genetic variation in FTO is associated with diminished satiety[J]. J Clin Endocrinol Metab, 2008, 93(9):3640-3643.
[16] JOHNSON L, VAN JAARSVELD C H M, EMMETT P M, et al. Dietary energy density affects fat mass in early adolescence and is not modified by FTO variants[J]. PLoS One, 2009, 4(3):e4594.
[17] CECIL J E, TAVENDALE R, WATT P. An obesity-associated FTO gene variant and increased energy intake in children[J]. N Engl J Med, 2008, 359(24):2558-2566.
[18] WÅHLÉN K, SJÖLIN E, HOFFSTEDT J. The common rs9939609 gene variant of the fat mass-and obesity-associated gene FTO is related to fat cell lipolysis[J]. J Lipid Res, 2008, 49(3):607-611.
[19] LI H, KILPELÄINEN T O, LIU C, et al. Association of genetic variation in FTO with risk of obesity and type 2 diabetes with data from 96,551 East and South Asians[J]. Diabetologia, 2012, 55(4):981-995.
[20] KIM J H, LEE J, OH B, et al. Prediction of phosphorylation sites using SVMs[J]. Bioinformatics, 2004, 20(17):3179-3184.
[21] GUPTA R, BRUNAK S. Prediction of glycosylation across the human proteome and the correlation to protein function[J]. Pac Symp Biocomput, 2002, 7:310-322.
[22] HAN Z F, NIU T H, CHANG J B, et al. Crystal structure of the FTO protein reveals basis for its substrate specificity[J]. Nature, 2010, 464(7292):1205-1209.
[23] OZER A, BRUICK R K. Non-heme dioxygenases:cellular sensors and regulators jelly rolled into one?[J]. Nat Chem Biol, 2007, 3(3):144-153.
[24] 孙春亮, 季兵, 付海波, 等. 脾切除对脂质代谢影响的临床研究[J]. 肝胆胰外科杂志, 2002, 14(3):154-156.
SUN C L, JI B, FU H B, et al. Clinical study of the effect of splenectomy on lipid metabolism[J]. Journal of Hepatopancreatobiliary Surgery, 2002, 14(3):154-156. (in Chinese)
[25] 林亚秋, 廖红海, 贺庆华, 等. 山羊FTO基因克隆及其表达谱[J]. 畜牧兽医学报, 2016, 47(5):888-898.
LIN Y Q, LIAO H H, HE Q H, et al. Cloning and expression profiling of FTO gene of goat[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(5):888-898. (in Chinese)
[26] CHEN X L, ZHOU B, LUO Y L, et al. Tissue distribution of porcine FTO and its effect on porcine intramuscular preadipocytes proliferation and differentiation[J]. PLoS One, 2016, 11(3):e0151056.
[27] SONG C, SONG W T, SHU J T, et al. Tissue-and breed-specific expression of the chicken fat mass-and obesity-associated gene (FTO)[J]. Genet Mol Res, 2015, 14(3):10500-10506.
[28] JEVSINEK SKOK D, KUNEJ T, KOVAC M, et al. FTO gene variants are associated with growth and carcass traits in cattle[J]. Anim Genet, 2016, 47(2):219-222.
[29] CHUNG E R. Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)[J]. J Anim Sci Technol, 2014, 56:27.
[30] FONTANESI L, SCOTTI E, BUTTAZZONI L, et al. Confirmed association between a single nucleotide polymorphism in the FTO gene and obesity-related traits in heavy pigs[J]. Mol Biol Rep, 2010, 37(1):461-466.
[31] DVORÁKOVÁ V, BARTENSCHLAGER H, STRATIL A, et al. Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses[J]. Genet Sel Evol, 2012, 44:13.
[32] 徐心祥, 刘峰. FTO基因在3T3-L1前体脂肪细胞成脂分化中的表达趋势[J]. 实用临床医药杂志, 2009, 13(11):76-77.
XU X X, LIU F. Expression of FTO gene in adipogenic differentiation of 3T3-L1 preadipocytes[J]. Journal of Clinical Medicine in Practice, 2009, 13(11):76-77. (in Chinese)
[33] 牛丛丛. 脂肪和肥胖相关基因(FTO)的转录调控及其功能研究[D]. 武汉:华中农业大学, 2012.
NIU C C. The transcriptional regulation and functional research of fat mass and obesity associated gene FTO[D]. Wuhan:Huazhong Agricultural University, 2012. (in Chinese)

Temporal-Spatial Characteristics of FTO Gene Expression in Tibetan Chicken and Its Correlation with Intramuscular Fat Content

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TANG Xinxin, ZHENG Jumei, LUO Na, YING Fan, ZHU Dan, LI Sen, LIU Dawei, AN Bingxing, WEN Jie, ZHAO Guiping, LI Hegang. Genetic Mechanism of Broiler Leg Disease Based on Genome-Wide Association Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 99-109.
[2]	XU Xiaojing, DONG Ruiling, WEI Limin, ZHAO Shaomeng, ZHAO Guiping, ZHANG Minhong, FENG Jinghai. Study on Growth Model of Wenchang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4952-4961.
[3]	QI Lina, LU Xuelin, YANG Kaixuan, ZHOU Jin, LI Xianyu, LIU Ke, ZHANG Wengang, GU Xin, ZHANG Weijian. Analysis of Genetic Diversity and Genetic Structure of New Pudong Chicken Based on SNP Chips [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4962-4971.
[4]	WANG Yanxing, ZHANG Yushi, JI Haigang, LIU Yang, NIU Yufang, HAN Ruili, LIU Xiaojun, TIAN Yadong, KANG Xiangtao, LI Zhuanjian. Establishment and Analysis of the Chicken Skeletal Satellite Cell Line [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4972-4981.
[5]	FAN Chenyu, SHAN Yanju, ZHANG Ming, JI Gaige, JU Xiaojun, TU Yunjie, HE Xi, SHU Jingting, LIU Yifan, ZHANG Haihan. Genome-wide Association Study of Body Weight and Meat Quality Traits in Lihua Mahuang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4982-4992.
[6]	CHEN Cheng, QIAO Xibo, SUN Yi, KANG Li, JIANG Yunliang. Study on the Polymorphism and Genetic Effect of the -868 Locus of FSHR Gene on Egg Laying Traits in Langya Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4560-4568.
[7]	JIN Sihua, JIA Fumin, HE Peili, JIA Yuqing, SHUI Fei, LIU Xuling, LIU Xing, WANG Xin, XU Man, GENG Zhaoyu. Effect of IHH Gene Overexpression on Proliferation and Differentiation of Chicken Primary Chondrocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4569-4576.
[8]	CHEN Xuejiao, LIU Huijie, ZANG Lei, FENG Jing, PENG Da, ZHANG Hao. Transcriptome Data from Chicken Embryo Heart Tissue Identified Key Genes for Altitude Hypoxia Adaptation in Xueyu White Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4154-4163.
[9]	ZHANG Yinliang, YUE Qiaoxian, HUANG Chenxuan, CHEN Hui, WANG Dehe, ZHOU Rongyan. Construction of Tibia Transcript Profile of Laying Hens at the Early and Late Laying Stages and Analysis of Genes Related to Bone Metabolism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4164-4173.
[10]	ZHENG Gang, LIAN Ling. Research Progress of Key Regulatory Gene DMRT1 in Chicken Sex Determination and Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3152-3163.
[11]	BAI Lu, WANG Mengjie, MA Xiaochun, HE Zhengxiao, TAN Xiaodong, LIU Jie, ZHAO Guiping, WEN Jie, LIU Ranran. A Method for Quickly Mining the Characteristic SNP Markers Set of Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3252-3261.
[12]	CHEN Chun, KANG Zhaofeng, WEI Yue, LI Guanhong, WU Yanping, XIE Jinfang. Correlation of Wnt3a Gene Polymorphism with Skin Follicle Traits in Chongren Partridge Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2810-2823.
[13]	LIU Hang, WANG Huanhuan, GE Ying, ZHANG Lei, ZHANG Weiwu, WEI Yinghui, LI Qinghai, FAN Jinghui, ZHANG Xuedong. Screening of Candidate Genes of Skin Color of Black-Bone Chicken Based on Transcriptome and Proteome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2320-2329.
[14]	WANG Hui, XU Mengyuan, LIU Lingkang, ZHENG Xibang, LI Gonghe, WU Wende. PhiC31 Integrase Triggers Site-specific Cassette Exchange in Chicken Fibroblast Cells through Electroporation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2330-2342.
[15]	WANG Zheng, GUO Wenjie, CHENG Jin, YUAN Yitong, LUO Rong, XUE Yi, ZHANG Lihuan, ZHU Zhiwei, LI Huifeng. Association Study of Polymorphism in the Regulatory Region of Nutrient Transport-related Genes and Feed Conversion Ratio in Yellow-feather Broiler [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2343-2352.